garlic extract can induce apoptotic cell death in the human colon adenocarcinoma ht29 cell line
Authors
abstract
background and objective: garlic has been known worldwide as a dietary constituent with many pharmacological effects. the present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. material and methods: human colon adenocarcinoma cells ht29 were treated with different doses (1, 5 and 10 mg/ml) of fresh garlic extract in cell culture at 24, 48 and 72 h. cell death was assessed by viability test, and its apoptotic nature was determined by terminal deoxynucleotidyltransferase-mediated dutp nick end labeling (tunel) assay. all experiments were statistically analyzed using spss 11.5 software results: garlic extract induced a dose-dependent cell death with the highest values at 72 hours. the apoptosis analyses by tunel assay demonstrated that in each dose/time group the apoptotic values are much less prominent than the related cell death percentage found in viability test. in every dose, the sum of apoptotic cells in all three-time points was nearly equal to the percentage of cell death at 72 hours. conclusion: fresh garlic extract can induce a dose-dependent apoptotic cell death in cultured ht29 cell line.
similar resources
Garlic Extract Can Induce Apoptotic Cell Death in The Human Colon Adenocarcinoma HT29 Cell Line
Background and Objective: Garlic has been known worldwide as a dietary constituent with many pharmacological effects. The present in vitro study was designed to investigate the putative anticancer effect of garlic extract on cancer cells, and if this effect was through apoptosis induction. Material and Methods: Human colon adenocarcinoma cells HT29 were treated with different doses (1, 5 a...
full textThe Pro-apoptotic Effect of Allicin on Human Colon Adenocarcinoma Cell Line HT29
Background and Objective: The management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. In the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line HT29 as an apoptosis resistant cell line, i...
full textthe pro-apoptotic effect of allicin on human colon adenocarcinoma cell line ht29
background and objective: the management of apoptotic cell death has been considered as a putative therapeutic strategy for cancer treatment. in the present study we investigated the putative pro-apoptotic effect of allicin, the main garlic organosulfur component with repeatedly claimed chemopreventive potency, on the human adenocarcinoma cell line ht29 as an apoptosis resistant cell line, in v...
full textInduced differentiation in HT29, a human colon adenocarcinoma cell line.
The human colon adenocarcinoma cell line HT29 displays an undifferentiated phenotype under standard growth conditions. When these cells were cultured for 21 days and then treated with forskolin, most of the cells formed brush borders on their apical surfaces. Brush border formation was inhibited by cytochalasin D but not by colchicine. Colchicine, nocodazole and taxol were found to induce diffe...
full textRutamarin, an Active Constituent from Ruta angustifolia Pers., Induced Apoptotic Cell Death in the HT29 Colon Adenocarcinoma Cell Line
BACKGROUND Ruta angustifolia Pers. is a perennial herb that is cultivated worldwide, including Southeast Asia, for the treatment of various diseases as traditional medicine. OBJECTIVE The purpose of the study was to identify an active principle of R. angustifolia and to investigate its effect on the HT29 cell death. MATERIALS AND METHODS The methanol and fractionated extracts (hexane, chlor...
full textEffect of citric acid on Bioavailability and apoptosis induction in human colorectal Adenocarcinoma cell line (HT29)
Background and Aim: Colorectal adenocarcinoma is one of the common causes of death due to weak response to common therapies. In this study, the effect of citric acid on bioavailability and apoptosis of the human colorectal adenocarcinoma cell line (HT29) was examined. Citric acid is a naturally organic acid that commonly found in citrus and is considered as a physiological inhibitor of enzymes ...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of pathologyPublisher: iranian society of pathology
ISSN 1735-5303
volume 5
issue 3 2010
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023